Thermo Fisher Scientific Phospho-IRS1 (Ser616) Recombinant Superclonal Antibody (26HCLC)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody is predicted to react with Monkey, Bovine, Horse, and Cat.

Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They consist of multiple recombinant monoclonal antibodies, combining the sensitivity of polyclonal antibodies with the specificity and consistency of monoclonal antibodies.

These antibodies recognize multiple epitope sites, providing higher detection sensitivity for low-abundance targets. Each lot is produced with a defined mixture of light and heavy chains, eliminating the variability associated with traditional polyclonal antibodies.

Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and performance remain unchanged.

Target Information

IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to insulin, IGF-1, and IL-4 stimulation. It undergoes phosphorylation on serine, threonine, and tyrosine residues across various tissues.

Casein kinase II mediates part of the insulin-stimulated serine/threonine phosphorylation of IRS-1, with Thr502 and Ser99 identified as major phosphorylation sites in rat IRS-1. These phosphorylation events may be part of the intracellular insulin signaling cascade.

IRS-1 also contains three putative 14-3-3 binding sites (Ser270, Ser374, Ser641), with the Ser270 motif located within the phosphotyrosine-binding domain responsible for interaction with the insulin receptor.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.