Thermo Fisher Scientific Phospho-ERK3 (Ser189) Polyclonal Antibody
Phospho-ERK3 (Ser189) 특이적 폴리클로날 항체로, 인산화된 MAPK6/ERK3 검출에 사용됩니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 사람·마우스·랫에서 반응합니다. 고순도 정제 및 안정적 액상 형태로 제공됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAPK6 (Accession Q16659), corresponding to amino acid residues around phosphorylated Ser189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817094 |
Product Specific Information
This antibody detects endogenous levels of MAPK6/ERK3 only when phosphorylated at Ser189.
Target Information
The protein encoded by this gene belongs to the Ser/Thr protein kinase family and is closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases, also known as extracellular signal-regulated kinases (ERKs), are activated through phosphorylation cascades and serve as integration points for multiple biochemical signals.
This kinase is localized in the nucleus and is activated in fibroblasts upon treatment with serum or phorbol esters.
Tissue specificity: Highest expression in skeletal muscle, followed by brain. Also found in heart, placenta, lung, liver, pancreas, kidney, and skin fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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