Thermo Fisher Scientific MARVELD2 Polyclonal Antibody
MARVELD2(Tricellulin) 단백질을 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. Human, Mouse, Rat 반응성 확인. 항원 친화 크로마토그래피로 정제된 액상 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.25–3 µg/mL | 14 |
| Immunohistochemistry (IHC) | – | 4 |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL | 6 |
| Flow Cytometry (Flow) | 3–5 µg/1x10⁶ cells | – |
| Immunoprecipitation (IP) | – | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Dog, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein derived from the C-terminal region of the human Tricellulin protein (Accession# NP_001033692, Q8N4S9), identical to rhesus monkey and chimpanzee sequences; 89% homologous to rat and mouse; 88% to bovine and canine; 86% to horse sequence |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533851 |
Product Specific Information
This antibody specifically recognizes the Tricellulin/MARVELD-2 protein.
In Western blot, it detects a band at approximately 64 kDa.
Confirmed reactivity with:
- Human Caco-2 cells
- Dog MDCK cells
- Mouse IMCD-3 cells
- Mouse kidney lysates (WB)
- Mouse MTE7b cells (ICC)
Based on sequence homology, reactivity with rhesus monkey, chimpanzee, rat, bovine, and horse is also expected.
Target Information
Tight junctions form a crucial barrier for paracellular transport in epithelial cells. Tricellulin is the first protein identified that specifically localizes to tricellular tight junctions where three cells meet.
It has four membrane-spanning domains similar to claudins and is highly expressed in epithelial tissues such as the small intestine, kidney, and lung.
Functional studies using siRNA have shown that suppression of tricellulin compromises epithelial barrier integrity and tight junction formation.
Mutations in tricellulin that disrupt its interaction with the scaffolding protein ZO-1 highlight the importance of protein–protein interactions in maintaining tight junction function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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