Thermo Fisher Scientific RNF144B Monoclonal Antibody (OTI9H10), TrueMAB
인간 RNF144B 단백질을 인식하는 Mouse monoclonal antibody. Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose)로 안정화. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9H10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–256 of human RNF144B produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
RNF144B is an E3 ubiquitin-protein ligase that transfers ubiquitin from E2 enzymes (UBE2L3, UBE2L6) to target substrates such as LCMT2, promoting their degradation. It induces apoptosis via a p53-dependent, caspase-independent mechanism. Overexpression decreases the ubiquitin-dependent stability of BAX, leading to protection from cell death, though RNF144B is not inherently anti-apoptotic.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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