Thermo Fisher Scientific IgD Monoclonal Antibody (IA6-2), NovaFluor Blue 610-30S, eBioscience

Thermo Fisher Scientific IgD Monoclonal Antibody (IA6-2), NovaFluor Blue 610-30S

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Brilliant Violet 480
• Brilliant Violet 650
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 660-40S
• NovaFluor Blue 690
• NovaFluor Blue 725
• NovaFluor Blue 760
• NovaFluor Blue 800
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• NovaFluor Yellow 810
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD). IgD is co-expressed with membrane IgM on mature naive B cells and also exists as a soluble form in serum. It associates with the B cell receptor and mediates signal transduction upon antigen binding.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells. Recommended use at 5 µL (0.6 µg) per test (100 µL final volume, 10^5–10^8 cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample prior to combining antibodies with cells. For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor conjugates are based on Phiton™ technology using nucleic acid dye structures engineered for minimal spectral spillover and spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

IgD is a type I transmembrane protein co-expressed with IgM on mature naive B cells and found in soluble form in serum. It interacts with the B cell receptor and contributes to signal transduction upon antigen binding.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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