Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Yellow 730, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), NovaFluor Yellow 730

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661, 737, 805
• Brilliant Violet 480
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S, Blue 660-120S, Red 710, Red 725, Yellow 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG (50–80 kDa).
CD16 includes two similar genes:

• CD16A (FcγRIIIA): hetero-oligomeric, expressed in macrophages and NK cells
• CD16B (FcγRIIIB): monomeric, GPI-anchored, expressed in neutrophils

Polymorphisms NA-1 and NA-2 affect phagocytic capacity. CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, and triggers ADCC, phagocytosis, degranulation, and proliferation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample or per 100 µL of cell sample for single-color controls.
  • Recommended for all NovaFluor antibody panels.

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 552 nm
Emission: 718 nm
Laser: 561 nm (Yellow)

Target Information

CD16 (FcγRIII) encodes a receptor that recognizes the Fc portion of IgG, involved in immune complex clearance and antibody-dependent cytotoxicity.

• FCGR3A: expressed on NK cells, transmembrane form
• FCGR3B: expressed on neutrophils, PI-anchored form

Mutations are linked to:

• Immunodeficiency 20
• Recurrent viral infections
• Systemic lupus erythematosus
• Alloimmune neonatal neutropenia

Alternative splicing produces multiple isoforms.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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