Thermo Fisher Scientific BirA Polyclonal Antibody

Applications

Tested Dilution

Publications

ELISA (ELISA)

1:5,000-1:10,000

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant E. coli BirA/Bifunctional protein BirA protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787583

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for E. coli BirA/Bifunctional protein BirA.

Target Information

BirA Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5-adenylate (BirA-bio-5-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.