Thermo Fisher Scientific PDCD1 Monoclonal Antibody (OTI4F10), TrueMAB
PDCD1 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry에 사용 가능. 인간 시료 반응성. 리오필라이즈 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 면역억제 관련 PD-1 연구용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:900 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F10 |
| Immunogen | Full-length human recombinant PDCD1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PDCD1 (Programmed Cell Death 1, PD-1) plays a key role in regulating T-cell mediated immune responses.
Activated T cells express PD-1 receptors that deliver inhibitory signals upon binding to ligands PDL-1 or PDL-2 on antigen-presenting cells, thereby limiting immune activation.
PD-1 signaling contributes to immune tolerance and prevention of autoimmunity.
In SDS-PAGE analysis, PD-1 may migrate at a higher molecular weight than predicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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