Thermo Fisher Scientific MIF Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Published species

Chicken, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant rat MIF fusion protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2533277

Product Specific Information

36-7401 detects MIF from human, mouse and rat samples.

36-7401 has been successfully used in Western blot, immunohistochemical, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing MIF. Immunofluorescent staining in U2OS cells and Immunohistochemical staining of MIF in rat epididymis tissue with 36-7401. yields a staining pattern consistent with MIF in nucleus and cytosol.

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.