Thermo Fisher Scientific ASL Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 289-464 of human ASL (NP_000039.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ASL,uniProtId:P04424-1,ncbiNodeId:9606,antigenRange:289-464,antigenLength:464,antigenImageFileName:PA5-120992_ASL_P04424-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120992_ASL_P04424-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.84 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914564

Product Specific Information

Positive test controls include: HeLa, HepG2, SH-SY5Y, Mouse liver, Mouse brain, Mouse kidney, Rat liver.

Immunogen sequence: NPDSLELIRS KAGRVFGRCA GLLMTLKGLP STYNKDLQED KEAVFEVSDT MSAVLQVATG VISTLQIHQE NMGQALSPDM LATDLAYYLV RKGMPFRQAH EASGKAVFMA ETKGVALNQL SLQELQTISP LFSGDVICVW DYGHSVEQYG ALGGTARSSV DWQIRQVRAL LQAQQA

Target Information

ASL encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.