Thermo Fisher Scientific PolE3/p17 Polyclonal Antibody
Western blot, IHC, IP 등 다양한 응용에 적합한 PolE3/p17 폴리클로날 항체. 사람과 생쥐에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 4°C 보관, 1 mg/mL 농도. 연구용으로만 사용 가능.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 97 and 147 of Human DNA polymerase epsilon subunit 3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine, and Orangutan
Target Information
Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family and exists as two isoenzymes: sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. MtCK occurs in dimeric and octameric forms, unlike cytosolic creatine kinase isoenzymes, which are exclusively dimeric. Sarcomeric mitochondrial creatine kinase shares 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs shared among nuclear genes encoding mitochondrial proteins, potentially essential for coordinated activation during mitochondrial biogenesis. Three transcript variants encoding the same protein have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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