Thermo Fisher Scientific PolE3/p17 Polyclonal Antibody
Thermo Fisher Scientific의 PolE3/p17 폴리클로날 항체는 Western blot, IHC, IP 등 다양한 응용에 적합합니다. Rabbit IgG 기반으로 제작되었으며, Human DNA polymerase epsilon subunit 3(97–147) 영역을 면역원으로 사용합니다. 액상 형태로 제공되며 4°C에서 보관 가능합니다.
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Thermo Fisher Scientific PolE3/p17 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:5,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 97 and 147 of Human DNA polymerase epsilon subunit 3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted reactivity: Rat, Bovine, Orangutan (based on 100% sequence identity)
Target Information
Mitochondrial creatine kinase (MtCK) is responsible for transferring high-energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family and exists as two isoenzymes: sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. MtCK occurs in two different oligomeric forms—dimers and octamers—unlike the exclusively dimeric cytosolic isoenzymes. Sarcomeric MtCK shares approximately 80% homology with ubiquitous MtCK. This gene includes motifs common to nuclear genes encoding mitochondrial proteins, likely essential for coordinated activation during mitochondrial biogenesis. Three transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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