Thermo Fisher Scientific CD10 Monoclonal Antibody (eBioCB-CALLA (CB-CALLA)), APC, eBioscience

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: Not specified
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 8 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC-eFluor 780
• Biotin
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation available

Product Specific Information

The eBioCB-CALLA monoclonal antibody recognizes human CD10 (CALLA, NEP, enkephalinase, Neprilysin), a 100 kDa type II cell surface glycoprotein originally identified for its expression on most acute lymphoblastic leukemias (ALL). CD10 is identical to neutral endopeptidase (NEP) and functions as a Zn²⁺-dependent metallo-peptidase, regulating chemotactic and inflammatory processes involving neutrophils.

In B cells, CD10 plays a role in stromal cell-dependent B lymphopoiesis and is expressed in germinal center B cells. CD10 expression is also observed in enterocytes, renal tubules, and glomeruli. The eBioCB-CALLA and MEM-78 monoclonal antibodies partially block each other, suggesting recognition of similar epitopes.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry on normal human peripheral blood cells.
Recommended Usage: 5 µL (0.125 µg) per test in 100 µL final volume. Cell number range: 10⁵–10⁸ cells/test.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA) is a cell surface enzyme with neutral metalloendopeptidase activity that inactivates various biologically active peptides. It is expressed on lymphoblastic, Burkitt’s, and follicular germinal center lymphoma cells, as well as immature B cells in bone marrow and certain CML cells.

CD10 is also present in breast myoepithelial cells, bile canaliculi, fibroblasts, and highly expressed on kidney and gut epithelial brush borders. It cleaves peptides at hydrophobic residues, inactivating peptide hormones such as glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.

The CD10 gene encodes a 100 kDa type II transmembrane glycoprotein (>45 kb), with four alternatively spliced mRNA transcripts. CD10 dysfunction is associated with spinocerebellar ataxia 43 and Charcot-Marie-Tooth disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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