Thermo Fisher Scientific Mouse ICAD Synthetic Peptide

Applications

Control (Ctrl)

• Assay-dependent

Blocking Assay (BLOCK)

• Assay-dependent

Product Specifications

Product Specific Information

This peptide corresponds to 20 amino acid residues at the amino terminus of mouse ICAD.
PEP-0017 can be used as a blocking peptide with polyclonal antibody PA5-19889.

Target Information

ICAD is the inhibitor of caspase-3-activated DNase, which controls nuclear apoptosis.
It has two isoforms:

• ICAD-L/DFF45 (45 kDa): functional isoform
• ICAD-S/DFF35 (35 kDa): non-functional isoform

Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L is functional. ICAD is cleaved to allow caspase-activated DNase (CAD) to execute apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with ICAD. Activation occurs through caspase-3-mediated cleavage of ICAD at residues 117 and 224, producing three fragments released from CAD.

Beyond its DNase inhibitory role, ICAD acts as a CAD-specific folding chaperone. Recent studies suggest ICAD may be a potential target for restoring normal apoptotic signaling in colon and brain cancer cells. Two alternatively spliced transcript variants encoding distinct isoforms have been identified for the ICAD gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.