
Thermo Fisher Scientific Ly-49C/I/F/H Monoclonal Antibody (14B11), PE, eBioscience
마우스 NK 세포의 Ly-49C/I/F/H 수용체를 인식하는 단클론 항체로, Flow Cytometry에 적합합니다. PE 형광 표지로 565/576 nm에서 Excitation/Emission을 가지며, 0.2 mg/mL 농도의 액상 형태로 제공됩니다. 4°C 암소 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Syrian hamster / IgG |
| Recommended Isotype Control | Syrian Hamster IgG Isotype Control, PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14B11 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466106 |
Additional Formats
Product Specific Information
The 14B11 monoclonal antibody reacts with a subset of mouse Ly-49 receptors and recognizes a majority of mouse NK cells. This antibody binds to three putative inhibitory members of the Ly-49 family (F, I, and C) and one activating receptor, Ly-49H. 14B11 does not react with Ly-49A, B, D, or G. It has been reported to enhance the redirected lysis of FcR⁺ target cells by IL-2-activated NK cells.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test).
- Excitation: 488–561 nm
- Emission: 578 nm
- Laser Compatibility: Blue, Green, Yellow-Green Lasers
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
KLRA3 (Ly49C) is a member of the LY49 receptor family in Natural Killer (NK) cells that bind to MHC class I molecules. These receptors are classified as activating or inhibitory based on the presence of ITIM (inhibitory) or ITAM (activating) motifs. KLRA3 is considered an inhibitory receptor recognizing peptide-receptive H-2Kb.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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