Thermo Fisher Scientific Cullin 5 Polyclonal Antibody
Rabbit polyclonal antibody against Cullin 5 protein. Western blot 및 IHC(P)에서 검증됨. 사람, 마우스, 랫트 반응성. 동결건조 형태로 제공되며, 장기 보관 시 -20°C 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | – |
| Miscellaneous PubMed (Misc) | – | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the N-terminal of human Cullin 5 (Vasopressin activated calcium-mobilizing receptor 1) conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: Cullin 5.
Target Information
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate ubiquitination and subsequent proteasomal degradation of target proteins.
As a scaffold protein, it may contribute to catalysis by positioning the substrate and ubiquitin-conjugating enzyme. The functional specificity of the E3 complex depends on the variable substrate recognition component.
ECS(SOCS1) directs ubiquitination of JAK2 and may be involved in proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. It may also form a cell surface vasopressin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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