Thermo Fisher Scientific Shootin1 Polyclonal Antibody
Thermo Fisher Scientific의 Shootin1 Polyclonal Antibody는 Human, Mouse, Rat에 반응하며 WB, IHC, IP 실험에 적합합니다. Rabbit IgG 기반의 비결합형 항체로, 신경 극성화 및 신경돌기 성장 관련 연구에 활용됩니다. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 안정성을 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunoprecipitation (IP) | 1:100 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide surrounding Lys450 of human Shootin1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 30 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979429 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
SHTN1 is a protein-coding gene involved in generating internal asymmetric signals required for neuronal polarization and neurite outgrowth. It mediates netrin-1-induced F-actin-substrate coupling through activation of CDC42, RAC1, and PAK1-dependent signaling pathways, converting F-actin retrograde flow into traction forces that support filopodium extension and axon outgrowth.
SHTN1 also plays a role in cytoskeletal organization by regulating the localization of PI3K activity at the axonal growth cone and contributes to regenerative neurite outgrowth. In the developing cortex, it cooperates with KIF20B to promote the transition from multipolar to bipolar stages and radial migration of cortical neurons. It is involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in hippocampal neuron growth cones.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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