
Thermo Fisher Scientific V5 Tag Monoclonal Antibody (4C12E11), THE
V5 태그 단백질 검출용 마우스 단클론 항체로, Western blot, ICC/IF, Flow cytometry, ELISA, IP 등 다양한 응용에 사용 가능. KLH에 결합된 V5 태그 펩타이드로 면역된 하이브리도마에서 생산. 단백질 A로 정제된 동결건조 형태로 제공되며, 재구성 후 0.5 mg/mL 농도로 사용.
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Thermo Fisher Scientific V5 Tag Monoclonal Antibody (4C12E11), THE™
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–3 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg per 1×10⁶ cells |
| ELISA | 0.05–0.1 µg/mL |
| Immunoprecipitation (IP) | 1–5 µg/mL |
| Miscellaneous PubMed (Misc) | – |
Publications: View 6 publications
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4C12E11 |
| Immunogen | V5 tag peptide conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to achieve an antibody concentration of 0.5 mg/mL.
The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
V5 Tag Antibody is a useful tool for detecting recombinant proteins containing the V5 epitope.
THE™ V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mice immunized with V5 tag peptide conjugated to KLH.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize low abundance or poorly immunogenic proteins when specific antibodies are unavailable.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus SV5.
It is typically used with all 14 amino acids (GKPIPNPLLGLDST), though a shorter 9 amino acid sequence (IPNPLLGLD) is also used.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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