Thermo Fisher Scientific V5 Tag Monoclonal Antibody (4C12E11), THE

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

-

Immunocytochemistry (ICC/IF)

1-3 µg/mL

-

Flow Cytometry (Flow)

1-3 µg 1 x 10^6 cells

-

ELISA (ELISA)

0.05-0.1 µg/mL

-

Immunoprecipitation (IP)

1-5 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 6 publications 6 publications

Product Specifications

Species Reactivity

Tag

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4C12E11

Immunogen

V5 tag peptide conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• HRP
• iFluor 488
• iFluor 555
• iFluor 647
• Request custom conjugation

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

The V5 epitope tag (GKPIPNPLLGLDST) is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.THE™ V5 Tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with V5 tag peptide conjugated to KLH.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.