Thermo Fisher Scientific NAE1 Monoclonal Antibody (OTI1A10), TrueMAB
인간 NAE1 단백질을 인식하는 Mouse IgG2b 단클론 항체. Western blot에 적합하며, 재조합 단백질(aa 1-274)을 면역원으로 제작. 동결건조 형태, PBS(pH 7.3, 8% 트레할로스) 버퍼에 보관. -20°C에서 보관하며 재구성 후 약 1 mg/mL로 사용.
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Applications
- Western Blot (WB): Tested dilution 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–274 of human NAE1 produced in E. coli. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The NAE1 protein binds to the beta-amyloid precursor protein, which is a cell surface protein involved in signal transduction and possibly in the pathogenesis of Alzheimer’s disease.
NAE1 forms a heterodimer with UBE1C to bind and activate NEDD8, a ubiquitin-like protein, and is required for cell cycle progression through the S/M checkpoint.
Three transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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