Thermo Fisher Scientific CD22 Recombinant Rabbit Monoclonal Antibody (BLR214K)
Thermo Fisher Scientific의 CD22 Recombinant Rabbit Monoclonal Antibody (BLR214K)은 인간 CD22 단백질을 표적으로 하는 고특이성 항체로, WB, IHC, ICC, Flow Cytometry, IP 등에 사용 가능합니다. 재조합 단클론 항체로 높은 재현성과 신뢰성을 제공합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 0.05 µL/10⁶ cells |
| Immunoprecipitation (IP) | 12 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR214K |
| Immunogen | Recombinant protein representing the extracellular domain of Human B-cell receptor CD22 (residues 20–687) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 500 µg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunocytochemistry, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE cell sections.
Target Information
CD22 (BL-CAM) is a type I integral membrane glycoprotein with a molecular weight of 130–140 kDa. It is expressed in both the cytoplasm and cell membrane of B-lymphocytes, appearing early in B-cell differentiation at a similar stage as CD19.
Unlike other B-cell markers, CD22 membrane expression is restricted to mature B cells (CD22⁺) and is absent in plasma cells (CD22⁻), making it useful in phenotyping mature leukemias. CD22 is strongly expressed in hairy cell leukemia and is positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
CD22 preferentially binds to α2,6-linked sialic acid, and the recognition site can be masked by cis interactions on the same cell surface. Ligand-induced tyrosine phosphorylation during immune responses suggests a regulatory role in B-cell receptor signaling. CD22 may act as both a positive and negative regulator by interacting with Src family kinases or recruiting cytoplasmic phosphatases that inhibit signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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