Thermo Fisher Scientific KRT19 Monoclonal Antibody (OTI5H6), TrueMAB
인간 KRT19 단백질(aa 240-390)을 면역원으로 한 Mouse monoclonal antibody. Western blot 및 IHC(P) 검증됨. 무보존제, 동결건조 형태로 제공되며, PBS(8% trehalose) 완충액에 재구성 가능. 세포 분화 및 상피성 종양 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5H6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 240–390 of human KRT19 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, an additional desalting step is recommended (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Cytokeratin 19 is a member of the intermediate filament protein family, showing extensive biochemical diversity across human epithelial tissues.
Cytokeratins range in molecular weight from 40–68 kDa and have isoelectric points between 4.9–7.8.
Expression patterns of cytokeratin subtypes vary with epithelial type and differentiation stage, aiding in distinguishing epithelial malignancies.
Cytokeratin 19 is not expressed in hepatocytes, making it useful for identifying liver metastases.
Cytokeratin antibodies are valuable in immunohistochemistry, cytopathology, and flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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