Thermo Fisher Scientific Cdc34 Recombinant Rabbit Monoclonal Antibody (ARC1742)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1742

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-236 of human CDC34 (P49427) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CDC34,uniProtId:P49427-1,ncbiNodeId:9606,antigenRange:20-236,antigenLength:236,antigenImageFileName:MA5-38215_CDC34_P49427-1_Rabbit.svg,antigenImageFileNamePDP:MA5-38215_CDC34_P49427-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898132

Product Specific Information

Positive Samples: Jurkat, SH-SY5Y

Immunogen sequence: LQEEPVEGFR VTLVDEGDLY NWEVAIFGPP NTYYEGGYFK ARLKFPIDYP YSPPAFRFLT KMWHPNIYET GDVCISILHP PVDDPQSGEL PSERWNPTQN VRTILLSVIS LLNEPNTFSP ANVDASVMYR KWKESKGKDR EYTDIIRKQV LGTKVDAERD GVKVPTTLAE YCVKTKAPAP DEGSDLFYDD YYEDGEVEEE ADSCFGDDED DSGTEES

Target Information

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys-48-linked polyubiquitination. Cooperates with the E2 UBCH5C and the SCF (FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions Lys-21 and/or Lys-22 with a monoubiquitin. Cooperates with the SCF (SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.