Thermo Fisher Scientific PKA alpha Monoclonal Antibody
PKA alpha 단일클론 항체로 Western blot, IHC, ICC/IF, ELISA 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. 높은 특이성과 재현성을 제공하며 세포 신호전달 연구에 적합. 액상형, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:10,000–1:40,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| ELISA | 1 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein of human PKA C-alpha (PRKACA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2897777 |
Product Specific Information
Positive Samples: HeLa, PC-3, C6, Mouse testis, Mouse brain, Rat testis
Target Information
Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation, and behavior.
PKA consists of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates into a PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers.
The released PKAc can translocate into the nucleus and regulate multiple nuclear hormone receptors. PKAc-mediated activation of tonicity-dependent gene expression is cAMP-independent.
Humans express three types of PKAc subunit: PKAc alpha (present in most human tissues), PKAc beta, and PKAc gamma (found in testes).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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