Thermo Fisher Scientific CD31 (PECAM-1) Recombinant Rabbit Monoclonal Antibody (7O7K3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:200-1:600

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

7O7K3

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 630-738 of CD31/PECAM1 (NP_0004334) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD31 (PECAM-1),uniProtId:P16284-1,ncbiNodeId:9606,antigenRange:630-738,antigenLength:738,antigenImageFileName:MA5-37858_CD31_PECAM-1_P16284-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37858_CD31_PECAM-1_P16284-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.55 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2897778

Product Specific Information

Positive Samples: U-937, HUVEC, Jurkat, A-549

Immunogen sequence: AKQMPVEMSR PAVPLLNSNN EKMSDPNMEA NSHYGHNDDV RNHAMKPIND NKEPLNSDVQ YTEVQVSSAE SHKDLGKKDT ETVYSEVRKA VPDAVESRYS RTEGSLDGT

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.