Thermo Fisher Scientific CBX4 Monoclonal Antibody (6C5G3)
인간 CBX4 단백질을 표적으로 하는 Mouse IgG1 단클론 항체. Western blot, IHC, Flow cytometry, ELISA 등 다양한 응용에 적합. Protein G로 정제된 액상 형태로 제공되며, 장기 저장 시 -20°C 보관 권장. 연구용으로만 사용 가능.
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Thermo Fisher Scientific CBX4 Monoclonal Antibody (6C5G3)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C5G3 |
| Immunogen | Purified recombinant fragment of human CBX4 (AA: 397–514) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787290 |
Product Specific Information
MA5-31666 has been tested in indirect ELISA.
Target Information
Polycomb group (PcG) proteins are chromatin-associated proteins essential for embryonic and adult stem cell self-renewal and maintenance. Two distinct human PcG complexes have been identified:
- Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1, and RING domain-containing proteins (RING1, RING2, BMI1).
- CBX4 (Chromobox protein homolog 4 or PC2) acts as a transcriptional suppressor and E3 SUMO-protein ligase facilitating SUMO1 conjugation.
Depletion of CBX4 decreases cellular resistance to ionizing radiation and implicates CBX4 in DNA damage response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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