Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), FITC, eBioscience
FITC 결합형 CD117 (c-Kit) 단클론 항체로 마우스 및 돼지 시료에 반응. 주로 유세포분석(Flow Cytometry)에 사용되며, 조혈전구세포 및 비만세포 표지에 적합. 0.5 µg/test 권장, 488 nm 여기/520 nm 방출, 4°C 암소 보관.
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- 11-1171-8x (3개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD117 (c-Kit) Monoclonal Antibody (2B8), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunocytochemistry (ICC/IF) | - | View 6 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 87 publications |
| Miscellaneous (PubMed) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Pig |
| Published Species | Fish, Gerbil, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), FITC |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B8 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465185 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737
- Brilliant Violet 421, 480, 605, 650, 786
- eFluor 450
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The 2B8 monoclonal antibody reacts with mouse CD117 (c-Kit receptor), also known as Steel factor receptor or stem cell factor receptor. This 145-kDa tyrosine kinase receptor is expressed by hematopoietic progenitor cells in mouse bone marrow, mast cells, and plays a key role in cell signaling and activation.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse bone marrow cells (≤0.5 µg/test, 100 µL final volume).
Cell number: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Optical Properties:
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
KIT (c-KIT) is a proto-oncogene encoding a type 3 transmembrane receptor for mast cell growth factor (MGF or stem cell factor, SCF). KIT regulates growth and activation of hematopoietic and non-hematopoietic cells. Mutations are linked to gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism.
Upon ligand binding, c-Kit dimerizes and autophosphorylates tyrosine residues (e.g., Tyr703), enabling Grb2 docking and activation of Ras/ERK signaling. SCF/c-Kit also activates PI3K, PLC-γ, and JAK/STAT pathways, essential for hematopoiesis, stem cell maintenance, and gametogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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