Thermo Fisher Scientific Hsp27 Monoclonal Antibody (5D12.A12)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Immunohistochemistry (IHC)

1-10 µg/mL

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5-1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5D12.A12

Immunogen

Human Hsp27

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5-1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein which functions as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays a role in stress resistance and actin organization. Through its molecular chaperone activity, HSP27 regulates numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins. Mutations in the gene can result in Charcot-Marie-Tooth disease 2F and Neuronopathy distal hereditary motor 2B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.