Thermo Fisher Scientific WDYHV1 Polyclonal Antibody
WDYHV1 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot 및 direct ELISA에 적합하며, Human과 Mouse 반응성. 고순도 Protein G 정제, 3 mg/mL 농도, PBS/glycerol buffer에 보존.
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- PA5117702
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- pk
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Applications
- Western Blot (WB): 1:500–1:2,000
- Tested in direct-ELISA
Publications
- [References not provided]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human Protein N-terminal glutamine amidohydrolase protein (1–205 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2902331 |
Product Specific Information
This antibody has been tested in direct-ELISA.
Target Information
WDYHV1 mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in the N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination, and degradation as specified by the N-end rule.
It does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. The enzyme does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence activity. In contrast, a proline at position 2 virtually abolishes deamidation of N-terminal glutamine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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