Thermo Fisher Scientific PUS7 Polyclonal Antibody
Thermo Fisher Scientific의 PUS7 Polyclonal Antibody는 인간, 마우스, 랫트에서 반응하며 Western blot 및 Immunocytochemistry에 사용 가능합니다. 재조합 인간 PUS7 단백질을 면역원으로 제작된 rabbit IgG 폴리클로날 항체로, 고순도 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunocytochemistry (ICC/IF)
- Tested Dilution: Not specified
- Publications: 1 publication available
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PUS7. Recombinant protein control fragment (Product #RP-92628). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2646152 |
Product Specific Information
Immunogen sequence:
PGFDVIYPKH KIQEAYREML TADNLDIDNM RHKIRDYSLS GAYRKIIIRP QNVSWEVVAY DDPKIPLFNT DVDNLEGKTP PVFASEGKYR ALKMDFSLPP STYATMAIRE VLKMDTSIKN QTQ
Highest antigen sequence identity to the following orthologs:
- Mouse: 95%
- Rat: 95%
Target Information
Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141, PubMed:30778726).
Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs); pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141).
Also catalyzes pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919).
In addition to mRNAs and tRNAs, binds other RNA types such as snRNAs, Y RNAs, and vault RNAs, suggesting broad pseudouridylation activity (PubMed:29628141).
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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