Thermo Fisher Scientific Cofilin Polyclonal Antibody
Cofilin 단백질을 인식하는 Rabbit Polyclonal Antibody로, Human, Mouse, Rat에 반응합니다. WB, IHC, ICC 등 다양한 응용에 사용 가능하며, 고순도 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, 장기 보관 시 -20°C에서 안정적입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View publication |
| Immunohistochemistry (IHC) | - | View publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | View publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | View publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–166 of Human Cofilin 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545103 |
Product Specific Information
- Recommended positive controls: 293T, A431, NIH-3T3, PC-12, Rat2
- Predicted reactivity: Mouse (98%), Rat (99%), Pig (99%), Sheep (99%), Rhesus Monkey (99%), Bovine (99%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. It regulates actin cytoskeleton dynamics and is important for normal progress through mitosis and cytokinesis. Plays a role in cell morphology and cytoskeletal organization. Required for up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing efficiency in chemokine uptake and degradation. Also required for neural tube morphogenesis and neural crest cell migration.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information: www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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