Thermo Fisher Scientific APOBEC3C Polyclonal Antibody
Human APOBEC3C 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IP에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용.
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Thermo Fisher Scientific APOBEC3C Polyclonal Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:500–1:3,000
- Immunoprecipitation (IP): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–190 of Human APOBEC3C |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545105 |
Product Specific Information
- Recommended positive controls: NCI-H929
- Predicted reactivity: Rhesus Monkey (84%), Chimpanzee (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
APOBEC (apolipoprotein B mRNA editing enzyme, catalytic) proteins inhibit retroviruses by deaminating cytosine residues of viral RNA and DNA. The seven APOBEC3 genes or pseudogenes are found in a cluster resulting from gene duplication on chromosome 22.
Like APOBEC3G, APOBEC3F deaminates deoxycytosine to deoxyuracil in the minus strand of HIV-1 DNA, resulting in G to A hypermutation in the plus strand of DNA.
Thus, APOBEC3G and APOBEC3F provide a mechanism for innate immunity to retroviruses and contribute to sequence variation observed in many viruses.
Viral infectivity factor (Vif) imparts APOBEC3G and APOBEC3F resistance to HIV through impaired translation and accelerated degradation of APOBEC3 proteins by the 26S proteasome.
Interestingly, HIV-1 Vif cannot form a complex with APOBEC3G or APOBEC3F of mouse origin, making mouse APOBEC3G and APOBEC3F potent inhibitors of wildtype HIV-1 replication, whereas human APOBEC3G and APOBEC3F inhibit only Vif-deficient HIV-1 replication.
This implies that induction of APOBEC3G and APOBEC3F activity or blocking their interaction with Vif may provide a method for therapeutic intervention.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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