Thermo Fisher Scientific beta Tubulin Loading Control Monoclonal Antibody (BT7R), Alexa Fluor 647
Beta-Tubulin 단백질 검출용 Alexa Fluor 647 결합 단일클론 항체로, Western blot 및 면역세포염색에 적합합니다. 다양한 종에 반응하며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 신경세포 표지 및 로딩 컨트롤용으로 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:25–1:100 | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Non-human primate, Rabbit, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BT7R |
| Immunogen | KLH conjugated peptide of the N-terminus of Beta-Tubulin |
| Conjugate | Alexa Fluor™ 647 |
| Excitation/Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609651 |
Available Formats
- Unconjugated
- Alexa Fluor™ 488
- Alexa Fluor™ 555
- Biotin
- DyLight™ 680
- DyLight™ 800 4X PEG
- HRP
- Custom conjugation available
Target Information
Beta-tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize to form microtubules. Beta-III tubulin is primarily expressed in neurons and is involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta-tubulin gene can cause congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also found in Sertoli cells and transiently in non-neuronal embryonic tissues. Tubulin phosphorylation on Ser-172 by CDK1 regulates microtubule incorporation during cell cycle progression.
Because beta-tubulin is ubiquitously expressed in eukaryotic cells, it is widely used as a loading control in protein detection assays such as Western blotting.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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