Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), PE-Cyanine7, eBioscience
인간 TNF alpha를 인식하는 중화용 단클론 항체로 PE-Cyanine7 형광체가 결합되어 있습니다. Flow cytometry 및 intracellular staining에 최적화되어 있으며, 높은 특이성과 재현성을 제공합니다. 4°C에서 암소 보관하며, 빛에 민감합니다.
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Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), PE-Cyanine7, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 3 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | View 47 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MAb11 |
| Conjugate | PE-Cyanine7 |
| Excitation/Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_469686 |
Additional Formats Available:
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 650
- eFluor 450
- FITC
- PE
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha has cytolytic activity against various tumor cells and plays a key role in immune regulation. It forms dimers and trimers and also exists as a 26 kDa membrane-bound form. The MAb11 antibody is a neutralizing antibody.
Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per test (100 µL final volume).
Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
Excitation / Emission / Laser:
488–561 nm excitation; 775 nm emission; compatible with Blue, Green, and Yellow-Green lasers.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. Secreted mainly by macrophages, it binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha forms multimers and shows a single 17 kDa band under non-reducing SDS-PAGE conditions. Studies indicate its neuroprotective function and tumor necrosis activity. It plays a role in immune responses to infections and tumor cell necrosis and is implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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