Thermo Fisher Scientific IFN gamma Monoclonal Antibody (XMG1.2), PE-Cyanine7, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 660
• FITC
• Functional Grade
• PE
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The XMG1.2 antibody reacts with mouse interferon (IFN) gamma and functions as a neutralizing antibody. Mouse IFN gamma is a 20 kDa cytokine produced by activated T, B, and NK cells, exhibiting antiviral and antiparasitic activity. It synergizes with TNF alpha to inhibit cell proliferation and modulates immune responses through high-affinity receptors. Glycosylation does not affect its biological activity.

Applications Reported: Suitable for intracellular staining and flow cytometric analysis.
Applications Tested: Tested on Con A-stimulated mouse splenocytes at ≤0.25 µg/test (defined as antibody amount per 100 µL sample). Optimal titration recommended.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57). Samples can be stored up to 3 days at 4°C in the dark.
Excitation/Emission: 488–561 nm / 775 nm; suitable for Blue, Green, and Yellow-Green lasers.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is produced mainly by T-lymphocytes and NK cells in response to antigens and cytokines. It is a dimeric glycoprotein (~45 kDa), appearing as 25, 20, and 15.5 kDa bands due to glycosylation. Human IFN gamma does not cross-react with mouse IFN gamma. Functions include antiviral and antiproliferative activity, MHC induction, macrophage activation, and enhanced B-cell immunoglobulin secretion. It acts through IFN gamma receptor I/II and the JAK-STAT pathway. Expression is upregulated by IL2, FGF, EGF, and downregulated by vitamin D3 or DMN. Mutations in the IFN gamma gene are linked to aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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