Thermo Fisher Scientific IRF3 Monoclonal Antibody (OTI2G6), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:500

Immunocytochemistry (ICC/IF)

• Tested Dilution: Assay-Dependent

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

IRF3 (Interferon Regulatory Factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses.
It plays a critical role in innate immune defense against DNA and RNA viruses by regulating transcription of IFN-alpha, IFN-beta, and interferon-stimulated genes (ISG).
IRF3 is activated through phosphorylation by IKBKE and TBK1, leading to dimerization, nuclear localization, and association with CREB-binding protein to form DRAF1 complex, which activates type I IFN and ISG gene transcription.
Mutations in IRF3 can cause acute encephalopathy and herpes-specific infection-induced disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.