Thermo Fisher Scientific EBI3 (IL-27 subunit) Monoclonal Antibody (DNT27), eBioscience

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Description:
The monoclonal antibody DNT27 reacts with mouse Epstein-Barr Virus-induced gene 3 (EBI3). EBI3 is a 34 kDa soluble protein first identified in B cells that is upregulated after Epstein-Barr virus infection. It can form homodimers or heterodimerize with IL-27α (p28, IL-30) to create the cytokine IL-27, which is produced mainly by dendritic cells and macrophages. Additionally, EBI3 can form IL-35 by pairing with the p35 subunit of IL-12, produced by regulatory T cells.

Applications Reported:

• Suitable for use in immunoblotting (Western blot).

Applications Tested:

• Tested by Western blot using lysates from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells.
• Recommended concentration: ≤1–5 µg/mL.
• Optimize dilution for specific assay conditions.

Purity: Greater than 90% (SDS-PAGE)
Aggregation: Less than 10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein belonging to the hematopoietin receptor family. It plays a regulatory role in Th2-type immune responses and Th2-mediated tissue inflammation, possibly through modulation of iNKT cell function.
EBI3 forms heterodimers with IL-27 (via p28) and IL-35 (via p35), influencing immune signaling and cytokine regulation. It is widely expressed and regulated by TLR signaling via MyD88 and NF-κB pathways.
EBI3 suppresses IL-17 production while enhancing IL-10 expression, contributing to immune tolerance and inflammation control. It may also influence tumor metastasis and allergic airway inflammation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.