Thermo Fisher Scientific B7-H4 Polyclonal Antibody
Thermo Fisher Scientific의 B7-H4 Polyclonal Antibody는 인간 및 마우스 반응성을 가진 염소 유래 IgG 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. B7-H4 단백질의 면역 조절 기능 연구 및 암 관련 바이오마커 분석에 활용됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg per million cells | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse B7-H4 (Phe29–Pro258) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607079 |
Product Specific Information
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse B7-1, B7-2, B7-H1, B7-H2, B7-H3, and PD-L2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
B7-H4 (also known as B7x, VTCN1) is a costimulatory protein functioning as a negative regulator of T-cell mediated immunity. It delivers inhibitory signals to T-cells, preventing proliferation, cell cycle progression, and interleukin-2 production.
B7-H4 is expressed on activated T-cells, B-cells, monocytes, and dendritic cells. Aberrant expression is associated with lung, breast, and ovarian cancers.
Overexpression of B7-H4 may help tumor cells suppress T-cell immunity, facilitating tumor progression. VTCN1 may serve as a biomarker for early detection of ovarian cancer and renal cell carcinoma (RCC).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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