Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor Red 755, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.2 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available

Product Specific Information

Description:
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 forms heterodimers with alpha integrins (CD49a–f, CD51) to mediate cellular adhesion, trafficking, proliferation, and differentiation. These complexes bind extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin, and interact with receptors like VCAM-1 and MadCAM-1.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse bone marrow cells, ≤ 0.2 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability with NovaFluor dyes.
• Ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample before combining with cells.
• For single-color controls: use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red laser)

Target Information

Target: ITGB1 (Integrin Subunit Beta 1, CD29)
A 110 kDa cell surface glycoprotein expressed by various cell types, including all leukocytes. ITGB1 forms heterodimers with at least six alpha chains (α1–α6), determining binding properties of β1 (VLA) integrins. It functions as a cell adhesion molecule and is involved in platelet activation, immune cell trafficking, and extracellular matrix interactions.

Decreased ITGB1 expression correlates with tumor multidrug resistance. Upregulation occurs during leukocyte migration and inflammatory responses, including inflammatory bowel disease. ITGB1 also mediates cytokine secretion in airway smooth muscle cells. Two major isoforms exist: β1A (ubiquitous) and β1D (cardiac and skeletal muscle specific).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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