
Thermo Fisher Scientific PDIA6 Recombinant Rabbit Monoclonal Antibody (JF0974)
PDIA6 단백질을 표적으로 하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, Flow Cytometry에 적합합니다. 높은 특이성과 재현성을 제공하며, HEK293 세포에서 발현된 IgG 형 항체입니다. 단백질 A로 정제되었으며, 4°C 단기 보관 및 -20°C 장기 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JF0974 |
| Immunogen | Recombinant protein within PDIA6 aa 1–108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809787 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
P5 is a novel human protein with peptide-binding and chaperone activity specific to certain proteins. It shares structural similarities with PDI, an important ER protein, and contains an ER retention signal (KDEL).
P5 includes two CXXC-like motifs responsible for oxidoreductase activity, located at both termini. Studies indicate the N-terminal motif plays a key role in isomerase activity. Unlike most PDI-like proteins, P5 is not stress-inducible.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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