Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Brilliant Violet 711, eBioscience
인간 CD19 항원을 인식하는 HIB19 클론 단일클론 항체로, Brilliant Violet 711 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, B세포 표지 및 분석에 활용됩니다. 광민감성이 있으므로 암실 보관을 권장합니다.
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Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), Brilliant Violet 711, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 711 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIB19 |
| Conjugate | Brilliant Violet™ 711 |
| Excitation / Emission Max | 406 / 711 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2921030 |
Additional Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–737
- Brilliant Violet 480, 605, 650
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue, Red, Violet, Yellow Series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780
- Request custom conjugation available
Product Specific Information
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with BCR to amplify signaling.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for human peripheral blood cells at 5 µL (0.25 µg) per test.
Brilliant Violet™ 711 (BV711) emits at 713 nm and is suitable for cytometers equipped with a 405 nm violet laser. Use Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize non-specific polymer interactions.
Light Sensitivity: Protect from light due to photo-induced oxidation.
Fixation: Compatible with IC Fixation Buffer or 1-step Fix/Lyse Solution; not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR), regulating normal and malignant B cell proliferation. Mutations cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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