Thermo Fisher Scientific PLCG1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1202-1291 of human PLC gamma 1 (PLC gamma 1 (PLCG1)) (NP_0026512) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PLCG1,uniProtId:P19174-2,ncbiNodeId:9606,antigenRange:1202-1291,antigenLength:1291,antigenImageFileName:PA5-90632_PLCG1_P19174-2_Rabbit.svg,antigenImageFileNamePDP:PA5-90632_PLCG1_P19174-2_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.03 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806153

Product Specific Information

Immunogen sequence: LASLLIKIDI FPAKQENGDL SPFSGTSLRE RGSDASGQLF HGRAREGSFE SRYQQPFEDF RISQEHLADH FDSRERRAPR RTRVNGDNRL; Positive Samples: HeLa, HepG2, 293T, Mouse lung, Rat brain; Cellular Location: Cell projection, lamellipodium, ruffle

Target Information

The mammalian Phospholipase Cg (PLCg) family has two closely related proteins, PLCg1 and PLCg2. The PLCg isozymes have the core structure domains and a unique array of domains containing an additional PH domain, two SH2 domains and one SH3 domain. In response to extracellular stimuli, such as hormones and growth factors, receptor tyrosine kinases (RTKs) phosphorylate and activate PLCg. Activated PLCg catalyzes hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the metabolic second messenger molecules inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.