Thermo Fisher Scientific Zap-70 Polyclonal Antibody
Human ZAP-70 단백질을 인식하는 Goat Polyclonal Antibody로, Western Blot 및 Immunoprecipitation에 사용 가능. 액상 형태로 0.5 mg/mL 농도이며 TBS 버퍼(0.5% BSA, 0.02% NaN₃) 포함. T세포 및 NK세포 신호전달 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 0.3–1 µg/mL
Immunoprecipitation (IP)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence C-SDGYTPEPARITSPD |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2933507 |
Product Specific Information
- This antibody is expected to recognize isoform 1 (NP_001070.2) only.
- Tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer cells, playing a critical role in T-cell receptor activation.
Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region between the SH2 and kinase domains.
Phosphorylation at tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling, while tyrosine 292 phosphorylation terminates activation and attenuates signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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