Thermo Fisher Scientific SRC2 Polyclonal Antibody
SRC2 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, WB, IHC, ELISA에 적합합니다. 인간 및 랫트 반응성을 가지며 핵에서 주로 발현되는 SRC2 검출에 사용됩니다. 고순도 친화 크로마토그래피 정제, 액상 형태로 -20°C 보관.
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Thermo Fisher Scientific SRC2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 550–820 of human NCOA2 (NP_006531.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.23 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913651 |
Product Specific Information
- Positive test controls: 293T, BT-474
- Localization: Nucleus
- Immunogen sequence:
SSLASPDLKM GNLQNSPVNM NPPPLSKMGS LDSKDCFGLY GEPSEGTTGQ AESSCHPGEQ KETNDPNLPP AVSSERADGQ SRLHDSKGQT KLLQLLTTKS DQMEPSPLAS SLSDTNKDST GSLPGSGSTH GTSLKEKHKI LHRLLQDSSS PVDLAKLTAE ATGKDLSQES SSTAPGSEVT IKQEPVSPKK KENALLRYLL DKDDTKDIGL PEITPKLERL DSKTDPASNT KLIAMKTEKE EMSFEPGDQP GSELDNLEEI LDDLQNSQLP Q
Target Information
SRC2 (steroid receptor coactivator 2), also known as nuclear receptor coactivator 2, NCOA2 or TIF2, aids in the function of nuclear hormone receptors.
Nuclear hormone receptors are conditional transcription factors that regulate cell growth, development, and homeostasis by controlling expression of specific genes.
They are characterized by three domains:
- N-terminal transcriptional activation domain
- Central DNA-binding domain
- C-terminal hormone-binding domain
Before hormone binding, steroid receptors remain inactive in a complex with heat-shock protein-90 and other stress proteins.
Upon hormone binding, conformational changes occur, enabling the receptors to dissociate, form homodimers, bind to DNA enhancer elements, and modulate gene transcription.
Transcriptional coactivator proteins such as SRC2 are required to mediate transcription initiation after enhancer binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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