Thermo Fisher Scientific CaMKII alpha Polyclonal Antibody
Rabbit polyclonal antibody against human CaMKII alpha for WB, IHC, and ICC applications. Validated for neuronal and cellular signaling studies. Supplied unconjugated in PBS with glycerol; antigen affinity purified for high specificity.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:50 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human CAMK2A. Recombinant protein control fragment (Product #RP-104470). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2791236 |
Product Specific Information
Immunogen sequence: LYQQIKAGAY DFPSPEWDTV TPEAKDL
Target Information
CAMK2A (CaMKII alpha) is a serine/threonine kinase that regulates various cellular functions, including neuronal processes, gene expression, carbohydrate metabolism, cytoskeletal function, and calcium homeostasis.
Autophosphorylation of threonine 286 in the presence of Ca²⁺ and calmodulin activates CAMK2A, enabling calmodulin-independent activity.
CaMKII is ubiquitously expressed and involved in cell cycle regulation, transcription, dendritic spine formation, neuronal plasticity, and sarcoplasmic reticulum Ca²⁺ transport in skeletal muscle.
In neurons, it plays a structural role in actin cytoskeleton reorganization during plasticity by binding and bundling actin filaments in a kinase-independent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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