Thermo Fisher Scientific AKAP5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human AKAP5(Accession P24588), corresponding to amino acid residues M1-F37. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKAP5,uniProtId:P24588-1,ncbiNodeId:9606,antigenRange:1-37,antigenLength:427,antigenImageFileName:PA5-101095_AKAP5_P24588-1_Rabbit.svg,antigenImageFileNamePDP:PA5-101095_AKAP5_P24588-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2850539

Product Specific Information

Antibody detects endogenous levels of total AKAP5.

Target Information

AKAPs (A-kinase anchoring proteins), as their name implies, are a family of scaffolding proteins that bind regulatory subunits of Protein Kinase A (PKA) thus localizing PKA activity to distinct regions of the cell. Beyond a common amphipathic alpha helix that is responsible for recruiting the PKA regulatory subunit (RI alpha, RII alpha, RI beta, or RII beta), individual AKAPs contain additional domains responsible for the recrutiment of additional signaling proteins (phosphodiesterases, phosphatases, cytoskeletal components, other kinase, etc.) or restricting AKAP to a specific subcellular location. AKAP5 (also known as P75, AKAP75, or AKAP79) is predominantly expressed in neuronal tissues and cells where it serves to localize type II PKA to post-synaptic densities. AKAP5 specifically binds to the regulatory subunit of PKAII beta, anchoring the enzyme to the plasma membrane and sites of cytoskeletal/membrane junctions. The other binding domains of AKAP5 have been shown to interact with calmodulin, PP2B, and calcineurin suggesting that AKAP5 may act to coordinate the cAMP- and Ca2+-sensing pathways in various cell types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.