Thermo Fisher Scientific IL-2 Monoclonal Antibody (1A12), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

View 12 publications 12 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1A12

Immunogen

Recombinant mouse IL-2

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Caprylic acid precipitation

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223599

Product Specific Information

MM102B targets IL-2 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM102B immunogen is recombinant mouse IL-2.

MM102B detects IL-2 which has a predicted molecular weight of approximately 17 kDa.

The MM102B mouse IL2 antibody (biotinylated conjugate of clone 1A12) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM101 (clone 5H4). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody MM102B (clone 1A12) and antibody MM101 (clone 5H4) have successfully been used in combination with recombinant mouse IL2 protein SMIL2 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.