Thermo Fisher Scientific RHOA Polyclonal Antibody
RHOA 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot에 적합합니다. Human, Mouse, Rat 등 다양한 종에 반응하며, 친화 크로마토그래피로 정제되었습니다. PBS/50% glycerol 용액 형태로 -20°C에서 보관합니다.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human RHOA (Accession P61586), corresponding to amino acid residues T163–L193 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2900477 |
Product Specific Information
This antibody detects endogenous levels of total RhoA.
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It participates in microtubule-dependent signaling required for myosin contractile ring formation during cytokinesis and plays a key role in cleavage furrow formation.
RhoA is essential for apical junction formation in keratinocyte adhesion and serves as a target for Yersinia pestis and Yersinia pseudotuberculosis toxins. It stimulates PKN2 kinase activity and may activate PLCE1. Activated by ARHGEF2, RhoA promotes GDP-GTP exchange and is crucial for SPATA13-mediated regulation of cell migration and adhesion dynamics.
The MEMO1–RHOA–DIAPH1 signaling pathway contributes to ERBB2-dependent microtubule stabilization at the cell cortex, regulating APC and CLASP2 localization and enabling microtubule capture via MACF1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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