Thermo Fisher Scientific LDHA Monoclonal Antibody (OTI5G10), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+.
In mammals, LDH is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five LDH isoenzymes based on the proportion of M and H chains in the tetrameric structure.
LDHA expression is hypoxia-inducible and directly regulated by HIF1a transcriptional activity.
LDHB is also upregulated in various cancers.
Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.