Thermo Fisher Scientific LDHA Monoclonal Antibody (OTI5G10), TrueMAB
인간 LDHA를 인식하는 mouse IgG1 단클론 항체로, WB, IHC(P), IP에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도 유지. Glycolysis 관련 LDHA 연구 및 HIF1a 조절 메커니즘 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5G10 |
| Immunogen | Full length human recombinant protein of human LDHA produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Lactate dehydrogenase (LDH) is an enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+.
In mammals, LDH is encoded by three genes: LDHA (muscle form), LDHB (heart form), and LDHC (testis form).
Human LDHs share 84–89% sequence similarity and 69–75% amino acid identity.
There are five LDH isoenzymes based on the proportion of M and H chains in the tetrameric structure.
LDHA expression is hypoxia-inducible and directly regulated by HIF1a transcriptional activity.
LDHB is also upregulated in various cancers.
Mutations in LDHA can cause Glycogen Storage Disease 11 (GSD11).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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