Thermo Fisher Scientific Nucleolin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000-1:8,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

1:50-1:100

View 3 publications 3 publications

ChIP assay (ChIP)

1:50-1:100

-

Immunomicroscopy (IM)

1:10-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Plant, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fusion protein containing amino acids 284-709 of human nucleolin. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nucleolin,uniProtId:P19338-1,ncbiNodeId:9606,antigenRange:284-709,antigenLength:710,antigenImageFileName:PA3-16875_Nucleolin_P19338-1_Rabbit.svg,antigenImageFileNamePDP:PA3-16875_Nucleolin_P19338-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2150738

Product Specific Information

Heat mediated antigen retrieval is recommended for IHC on paraffin embedded tissues.

Suggested positive control: PD31 murine pre-B cells for immunofluorescence and crude PD31 nuclear extracts for Western Blot.

Target Information

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.