Thermo Fisher Scientific Nucleolin Polyclonal Antibody
Nucleolin 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot, IHC, ICC 등 다양한 응용에 적합합니다. 인간, 생쥐, 식물, 랫트 시료에 반응하며, 비결합형 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:4,000–1:8,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | View 3 publications |
| ChIP assay (ChIP) | 1:50–1:100 | - |
| Immunomicroscopy (IM) | 1:10–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Plant, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein containing amino acids 284–709 of human nucleolin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2150738 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended for IHC on paraffin-embedded tissues.
- Suggested positive control: PD31 murine pre-B cells for immunofluorescence and crude PD31 nuclear extracts for Western Blot.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in ribosome synthesis and maturation. It is primarily located in the dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA called U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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