Thermo Fisher Scientific SIRT2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:400

View 1 publication 1 publication

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Horse, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: D(341) E A R T T E R E K P Q(352) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SIRT2,uniProtId:Q8IXJ6-1,ncbiNodeId:9606,antigenRange:341-352,antigenLength:389,antigenImageFileName:PA3-200_SIRT2_Q8IXJ6-1_Rabbit.svg,antigenImageFileNamePDP:PA3-200_SIRT2_Q8IXJ6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2188496

Product Specific Information

PA3-200 detects human and rat SIRT2 and shows no cross-reactivity to other SIRT isoforms.

PA3-200 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~48 kDa protein representing SIRT2 from SAOS2 cells. PA3-200 can be used for immunocytochemistry procedures.

PA3-200 immunizing peptide corresponds to amino acid residues 341-352 from the human SIRT2 protein. This sequence is completely conserved between human and rat.

Target Information

The SIRT2 protein (also known as Sirtuin for Silent Mating Type Information 2-Homolog) is a NAD-dependent deacytylase (NDAC) that has been shown to control gene silencing, cell cycle, and DNA damage repair. It is believed that SIRT2 may act as a tumor suppressor in human gliomas and may also serve as a novel molecular marker for these cells. SIRT2 has also been shown to act as a redox sensor to help regulate muscle gene expression in response to food intake and exercise. SIRT2 acts in the phosphorylation cascade involving mitosis where SIRT2 is phosphorylated in late G2 phase, during M phase, and into cytokinesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.